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phosphatase inhibitor cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphatase inhibitor cell signaling
    Phosphatase Inhibitor Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protease+inhibitor+cocktail+cell+signaling/Protease%2FPhosphatase+Inhibitor+Cocktail/pm41875887-825-122-124
    Average 98 stars, based on 2066 article reviews
    phosphatase inhibitor cell signaling - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: Alzheimer's disease pathology degrades an NMDA receptor-dependent spontaneous activity pattern in cortico-hippocampal circuits.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER GeltrexTM Basement Membrane Matrix ThermoFisher Cat#A4000046803 701 SN syringe (10 μl) Hamilton Part#80339 UltraMicroPump3 (with SMARTouch Controller) World Precision Instruments Product code: UMP3T-I OmniDrill35 World Precision Instruments Cat#503598 3MTM VetbondTM Tissue Adhesive Fisher Scientific Product code: 17150156 Norland Optical Adhesive 81 Edmund Optics Cat#36-429 LED UV Spot Lamp Intertronics Part#IUV101 Protease Inhibitor cocktail Cell Signaling Cat#5871S cOmplete ULTRA protease inhibitor cocktail Roche Cat#05892970001 PhosSTOP phosphatase inhibitor cocktail Roche Cat#4906845001 Zirconium Beads Scientific Laboratory Supplies Product code: SLS1414 ll OPEN ACCESSArticle Neuron 114, 1–18.e1–e9, July 1, 2026 e3 Human cerebral organoid cultures KOLF2.1J iPSC (46, XY, ApoE3/3) was used as a control line in this study, together with an isogenic line engineered to carry homozygous APPV717I mutations, both were obtained from the iPSC Neurodegenerative Disease Initiative90 via The Jackson Laboratory (KOLF2.1J: JIPSC001000, APPV717I: JIPSC001202). iPSCs were grown in StemFlex (ThermoFisher) on Geltrex (ThermoFisher). .. Cerebral organoids were generated using previously described protocols91 using the STEMdiff kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Adhesive:

    Article Title: Alzheimer's disease pathology degrades an NMDA receptor-dependent spontaneous activity pattern in cortico-hippocampal circuits.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER GeltrexTM Basement Membrane Matrix ThermoFisher Cat#A4000046803 701 SN syringe (10 μl) Hamilton Part#80339 UltraMicroPump3 (with SMARTouch Controller) World Precision Instruments Product code: UMP3T-I OmniDrill35 World Precision Instruments Cat#503598 3MTM VetbondTM Tissue Adhesive Fisher Scientific Product code: 17150156 Norland Optical Adhesive 81 Edmund Optics Cat#36-429 LED UV Spot Lamp Intertronics Part#IUV101 Protease Inhibitor cocktail Cell Signaling Cat#5871S cOmplete ULTRA protease inhibitor cocktail Roche Cat#05892970001 PhosSTOP phosphatase inhibitor cocktail Roche Cat#4906845001 Zirconium Beads Scientific Laboratory Supplies Product code: SLS1414 ll OPEN ACCESSArticle Neuron 114, 1–18.e1–e9, July 1, 2026 e3 Human cerebral organoid cultures KOLF2.1J iPSC (46, XY, ApoE3/3) was used as a control line in this study, together with an isogenic line engineered to carry homozygous APPV717I mutations, both were obtained from the iPSC Neurodegenerative Disease Initiative90 via The Jackson Laboratory (KOLF2.1J: JIPSC001000, APPV717I: JIPSC001202). iPSCs were grown in StemFlex (ThermoFisher) on Geltrex (ThermoFisher). .. Cerebral organoids were generated using previously described protocols91 using the STEMdiff kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Protease Inhibitor:

    Article Title: Alzheimer's disease pathology degrades an NMDA receptor-dependent spontaneous activity pattern in cortico-hippocampal circuits.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER GeltrexTM Basement Membrane Matrix ThermoFisher Cat#A4000046803 701 SN syringe (10 μl) Hamilton Part#80339 UltraMicroPump3 (with SMARTouch Controller) World Precision Instruments Product code: UMP3T-I OmniDrill35 World Precision Instruments Cat#503598 3MTM VetbondTM Tissue Adhesive Fisher Scientific Product code: 17150156 Norland Optical Adhesive 81 Edmund Optics Cat#36-429 LED UV Spot Lamp Intertronics Part#IUV101 Protease Inhibitor cocktail Cell Signaling Cat#5871S cOmplete ULTRA protease inhibitor cocktail Roche Cat#05892970001 PhosSTOP phosphatase inhibitor cocktail Roche Cat#4906845001 Zirconium Beads Scientific Laboratory Supplies Product code: SLS1414 ll OPEN ACCESSArticle Neuron 114, 1–18.e1–e9, July 1, 2026 e3 Human cerebral organoid cultures KOLF2.1J iPSC (46, XY, ApoE3/3) was used as a control line in this study, together with an isogenic line engineered to carry homozygous APPV717I mutations, both were obtained from the iPSC Neurodegenerative Disease Initiative90 via The Jackson Laboratory (KOLF2.1J: JIPSC001000, APPV717I: JIPSC001202). iPSCs were grown in StemFlex (ThermoFisher) on Geltrex (ThermoFisher). .. Cerebral organoids were generated using previously described protocols91 using the STEMdiff kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Article Title: Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation
    Article Snippet: Plasma samples were assayed for measurement of noradrenaline (Cloud-Clone, Texas – USA) levels, using enzyme-linked immunosorbent assay (ELISA) kits according to standard instructions. .. Spleens were homogenized in 0.5 mL of PBS, protease inhibitor cocktail (Cell Signaling, Massachusetts, USA) and then centrifuged at 13,000 rpm for 20 min at 4 °C. ..

    Article Title: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-p21 Waf1/Cip1 recombinant (12D1E1) Cell Signaling Cat# 2947; RRID: AB_823586 Anti-p21 Waf1/Cip1 Monoclonal (F-5) Santa Cruz Biotechnology Cat# sc-6246; RRID: AB_628073 anti-p16 INK4A Abcam Cat# ab189034; RRID: AB_2737282 anti-Ccng2 Invitrogen Cat# PA5-100707; RRID: AB_2850211 anti-GAPDH Abcam Cat# ab9485; RRID: AB_307275 anti-Alix Abcam Cat# ab275377; RRID: AB_3644262 anti-CD63 Abcam Cat# ab134045; RRID: AB_2800495 anti-TSG101 Abcam Cat# ab125011; RRID: AB_10974262 anti-Calnexin Abcam Cat# ab22595; RRID: AB_2069006 anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 anti-Ago2 Abcam Cat# ab186733; RRID: AB_2713978 anti-g-H2AX Cell Signaling Cat# 9718; RRID: AB_647828 anti-H3K9me3 Abcam Cat# ab8898; RRID: AB_306848 HRP anti-rabbit IgG Cell Signaling Cat# 7074; RRID: AB_2099233 HRP anti-mouse IgG Cell Signaling Cat# 7076; RRID: AB_330924 anti-Rabbit IgG H&L (Alexa Fluor 594) Abcam Cat# ab150080; RRID: AB_2650602 anti-Rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab15008; RRID: AB_301569 Chemicals, peptides, and recombinant proteins Doxorubicin Sigma Cat# 25316-40-9 Fetal bovine serum Gibco Cat# 10091-148 ncTarget Medium Nuwacell Biotechnologies Co., Ltd. Cat# RP01020 DAPI ThermoFisher Cat# D1306 EDTA ThermoFisher Cat# AM9261 DMEM ThermoFisher Cat# 11965092 Xho I New England Biolabs Cat# R0146S Not I New England Biolabs Cat# R0189S TRIzol Vazyme Cat# R401-01 FuGENE HD Promega Cat# E2311 Lipofectamine 2000 Life Technology Cat# 11668019 Protease inhibitor cocktail Cell Signaling Cat# 5871 Collagenase I Sigma Cat# SCR103 Collagenase IV Sigma Cat# C4-BIOC Dispase Sigma Cat# D4693 Red blood cell lysis buffer BD Biosciences Cat# 555899 Critical commercial assays Senescence b-Galactosidase Staining Kit Cell Signaling Cat# 9860 Quantibody Mouse TH17 Array 1 RayBiotech Cat# QAM-TH17-1 miRNeasy Mini Kit Qiagen Cat# 217004 Dual-Luciferase Reporter Assay Kit Promega Cat# E1910 Mouse IL-1b ELISA Kit R&D systems Cat# MLB00C Mouse IFN-g ELISA Kit R&D systems Cat# MIF00 Mouse IL-6 ELISA Kit R&D systems Cat# M6000B Mouse TNF-a ELISA Kit R&D systems Cat# MTA00B (Continued on next page) e1 Cell Metabolism 37, 1–15.e1–e6, February 4, 2025 .. REAGENT or RESOURCES SOURCE IDENTIFIER Deposited data scRNA-seq data This paper GEO: GSE247076 Public scRNA-seq data Almanzar et al.29 GEO: GSE132042 Bulk RNA-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Ago2 Clip-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Data S1.

    Control:

    Article Title: Alzheimer's disease pathology degrades an NMDA receptor-dependent spontaneous activity pattern in cortico-hippocampal circuits.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER GeltrexTM Basement Membrane Matrix ThermoFisher Cat#A4000046803 701 SN syringe (10 μl) Hamilton Part#80339 UltraMicroPump3 (with SMARTouch Controller) World Precision Instruments Product code: UMP3T-I OmniDrill35 World Precision Instruments Cat#503598 3MTM VetbondTM Tissue Adhesive Fisher Scientific Product code: 17150156 Norland Optical Adhesive 81 Edmund Optics Cat#36-429 LED UV Spot Lamp Intertronics Part#IUV101 Protease Inhibitor cocktail Cell Signaling Cat#5871S cOmplete ULTRA protease inhibitor cocktail Roche Cat#05892970001 PhosSTOP phosphatase inhibitor cocktail Roche Cat#4906845001 Zirconium Beads Scientific Laboratory Supplies Product code: SLS1414 ll OPEN ACCESSArticle Neuron 114, 1–18.e1–e9, July 1, 2026 e3 Human cerebral organoid cultures KOLF2.1J iPSC (46, XY, ApoE3/3) was used as a control line in this study, together with an isogenic line engineered to carry homozygous APPV717I mutations, both were obtained from the iPSC Neurodegenerative Disease Initiative90 via The Jackson Laboratory (KOLF2.1J: JIPSC001000, APPV717I: JIPSC001202). iPSCs were grown in StemFlex (ThermoFisher) on Geltrex (ThermoFisher). .. Cerebral organoids were generated using previously described protocols91 using the STEMdiff kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Recombinant:

    Article Title: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-p21 Waf1/Cip1 recombinant (12D1E1) Cell Signaling Cat# 2947; RRID: AB_823586 Anti-p21 Waf1/Cip1 Monoclonal (F-5) Santa Cruz Biotechnology Cat# sc-6246; RRID: AB_628073 anti-p16 INK4A Abcam Cat# ab189034; RRID: AB_2737282 anti-Ccng2 Invitrogen Cat# PA5-100707; RRID: AB_2850211 anti-GAPDH Abcam Cat# ab9485; RRID: AB_307275 anti-Alix Abcam Cat# ab275377; RRID: AB_3644262 anti-CD63 Abcam Cat# ab134045; RRID: AB_2800495 anti-TSG101 Abcam Cat# ab125011; RRID: AB_10974262 anti-Calnexin Abcam Cat# ab22595; RRID: AB_2069006 anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 anti-Ago2 Abcam Cat# ab186733; RRID: AB_2713978 anti-g-H2AX Cell Signaling Cat# 9718; RRID: AB_647828 anti-H3K9me3 Abcam Cat# ab8898; RRID: AB_306848 HRP anti-rabbit IgG Cell Signaling Cat# 7074; RRID: AB_2099233 HRP anti-mouse IgG Cell Signaling Cat# 7076; RRID: AB_330924 anti-Rabbit IgG H&L (Alexa Fluor 594) Abcam Cat# ab150080; RRID: AB_2650602 anti-Rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab15008; RRID: AB_301569 Chemicals, peptides, and recombinant proteins Doxorubicin Sigma Cat# 25316-40-9 Fetal bovine serum Gibco Cat# 10091-148 ncTarget Medium Nuwacell Biotechnologies Co., Ltd. Cat# RP01020 DAPI ThermoFisher Cat# D1306 EDTA ThermoFisher Cat# AM9261 DMEM ThermoFisher Cat# 11965092 Xho I New England Biolabs Cat# R0146S Not I New England Biolabs Cat# R0189S TRIzol Vazyme Cat# R401-01 FuGENE HD Promega Cat# E2311 Lipofectamine 2000 Life Technology Cat# 11668019 Protease inhibitor cocktail Cell Signaling Cat# 5871 Collagenase I Sigma Cat# SCR103 Collagenase IV Sigma Cat# C4-BIOC Dispase Sigma Cat# D4693 Red blood cell lysis buffer BD Biosciences Cat# 555899 Critical commercial assays Senescence b-Galactosidase Staining Kit Cell Signaling Cat# 9860 Quantibody Mouse TH17 Array 1 RayBiotech Cat# QAM-TH17-1 miRNeasy Mini Kit Qiagen Cat# 217004 Dual-Luciferase Reporter Assay Kit Promega Cat# E1910 Mouse IL-1b ELISA Kit R&D systems Cat# MLB00C Mouse IFN-g ELISA Kit R&D systems Cat# MIF00 Mouse IL-6 ELISA Kit R&D systems Cat# M6000B Mouse TNF-a ELISA Kit R&D systems Cat# MTA00B (Continued on next page) e1 Cell Metabolism 37, 1–15.e1–e6, February 4, 2025 .. REAGENT or RESOURCES SOURCE IDENTIFIER Deposited data scRNA-seq data This paper GEO: GSE247076 Public scRNA-seq data Almanzar et al.29 GEO: GSE132042 Bulk RNA-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Ago2 Clip-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Data S1.

    Red Blood Cell Lysis:

    Article Title: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-p21 Waf1/Cip1 recombinant (12D1E1) Cell Signaling Cat# 2947; RRID: AB_823586 Anti-p21 Waf1/Cip1 Monoclonal (F-5) Santa Cruz Biotechnology Cat# sc-6246; RRID: AB_628073 anti-p16 INK4A Abcam Cat# ab189034; RRID: AB_2737282 anti-Ccng2 Invitrogen Cat# PA5-100707; RRID: AB_2850211 anti-GAPDH Abcam Cat# ab9485; RRID: AB_307275 anti-Alix Abcam Cat# ab275377; RRID: AB_3644262 anti-CD63 Abcam Cat# ab134045; RRID: AB_2800495 anti-TSG101 Abcam Cat# ab125011; RRID: AB_10974262 anti-Calnexin Abcam Cat# ab22595; RRID: AB_2069006 anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 anti-Ago2 Abcam Cat# ab186733; RRID: AB_2713978 anti-g-H2AX Cell Signaling Cat# 9718; RRID: AB_647828 anti-H3K9me3 Abcam Cat# ab8898; RRID: AB_306848 HRP anti-rabbit IgG Cell Signaling Cat# 7074; RRID: AB_2099233 HRP anti-mouse IgG Cell Signaling Cat# 7076; RRID: AB_330924 anti-Rabbit IgG H&L (Alexa Fluor 594) Abcam Cat# ab150080; RRID: AB_2650602 anti-Rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab15008; RRID: AB_301569 Chemicals, peptides, and recombinant proteins Doxorubicin Sigma Cat# 25316-40-9 Fetal bovine serum Gibco Cat# 10091-148 ncTarget Medium Nuwacell Biotechnologies Co., Ltd. Cat# RP01020 DAPI ThermoFisher Cat# D1306 EDTA ThermoFisher Cat# AM9261 DMEM ThermoFisher Cat# 11965092 Xho I New England Biolabs Cat# R0146S Not I New England Biolabs Cat# R0189S TRIzol Vazyme Cat# R401-01 FuGENE HD Promega Cat# E2311 Lipofectamine 2000 Life Technology Cat# 11668019 Protease inhibitor cocktail Cell Signaling Cat# 5871 Collagenase I Sigma Cat# SCR103 Collagenase IV Sigma Cat# C4-BIOC Dispase Sigma Cat# D4693 Red blood cell lysis buffer BD Biosciences Cat# 555899 Critical commercial assays Senescence b-Galactosidase Staining Kit Cell Signaling Cat# 9860 Quantibody Mouse TH17 Array 1 RayBiotech Cat# QAM-TH17-1 miRNeasy Mini Kit Qiagen Cat# 217004 Dual-Luciferase Reporter Assay Kit Promega Cat# E1910 Mouse IL-1b ELISA Kit R&D systems Cat# MLB00C Mouse IFN-g ELISA Kit R&D systems Cat# MIF00 Mouse IL-6 ELISA Kit R&D systems Cat# M6000B Mouse TNF-a ELISA Kit R&D systems Cat# MTA00B (Continued on next page) e1 Cell Metabolism 37, 1–15.e1–e6, February 4, 2025 .. REAGENT or RESOURCES SOURCE IDENTIFIER Deposited data scRNA-seq data This paper GEO: GSE247076 Public scRNA-seq data Almanzar et al.29 GEO: GSE132042 Bulk RNA-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Ago2 Clip-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Data S1.

    Staining:

    Article Title: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-p21 Waf1/Cip1 recombinant (12D1E1) Cell Signaling Cat# 2947; RRID: AB_823586 Anti-p21 Waf1/Cip1 Monoclonal (F-5) Santa Cruz Biotechnology Cat# sc-6246; RRID: AB_628073 anti-p16 INK4A Abcam Cat# ab189034; RRID: AB_2737282 anti-Ccng2 Invitrogen Cat# PA5-100707; RRID: AB_2850211 anti-GAPDH Abcam Cat# ab9485; RRID: AB_307275 anti-Alix Abcam Cat# ab275377; RRID: AB_3644262 anti-CD63 Abcam Cat# ab134045; RRID: AB_2800495 anti-TSG101 Abcam Cat# ab125011; RRID: AB_10974262 anti-Calnexin Abcam Cat# ab22595; RRID: AB_2069006 anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 anti-Ago2 Abcam Cat# ab186733; RRID: AB_2713978 anti-g-H2AX Cell Signaling Cat# 9718; RRID: AB_647828 anti-H3K9me3 Abcam Cat# ab8898; RRID: AB_306848 HRP anti-rabbit IgG Cell Signaling Cat# 7074; RRID: AB_2099233 HRP anti-mouse IgG Cell Signaling Cat# 7076; RRID: AB_330924 anti-Rabbit IgG H&L (Alexa Fluor 594) Abcam Cat# ab150080; RRID: AB_2650602 anti-Rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab15008; RRID: AB_301569 Chemicals, peptides, and recombinant proteins Doxorubicin Sigma Cat# 25316-40-9 Fetal bovine serum Gibco Cat# 10091-148 ncTarget Medium Nuwacell Biotechnologies Co., Ltd. Cat# RP01020 DAPI ThermoFisher Cat# D1306 EDTA ThermoFisher Cat# AM9261 DMEM ThermoFisher Cat# 11965092 Xho I New England Biolabs Cat# R0146S Not I New England Biolabs Cat# R0189S TRIzol Vazyme Cat# R401-01 FuGENE HD Promega Cat# E2311 Lipofectamine 2000 Life Technology Cat# 11668019 Protease inhibitor cocktail Cell Signaling Cat# 5871 Collagenase I Sigma Cat# SCR103 Collagenase IV Sigma Cat# C4-BIOC Dispase Sigma Cat# D4693 Red blood cell lysis buffer BD Biosciences Cat# 555899 Critical commercial assays Senescence b-Galactosidase Staining Kit Cell Signaling Cat# 9860 Quantibody Mouse TH17 Array 1 RayBiotech Cat# QAM-TH17-1 miRNeasy Mini Kit Qiagen Cat# 217004 Dual-Luciferase Reporter Assay Kit Promega Cat# E1910 Mouse IL-1b ELISA Kit R&D systems Cat# MLB00C Mouse IFN-g ELISA Kit R&D systems Cat# MIF00 Mouse IL-6 ELISA Kit R&D systems Cat# M6000B Mouse TNF-a ELISA Kit R&D systems Cat# MTA00B (Continued on next page) e1 Cell Metabolism 37, 1–15.e1–e6, February 4, 2025 .. REAGENT or RESOURCES SOURCE IDENTIFIER Deposited data scRNA-seq data This paper GEO: GSE247076 Public scRNA-seq data Almanzar et al.29 GEO: GSE132042 Bulk RNA-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Ago2 Clip-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Data S1.

    Reporter Assay:

    Article Title: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-p21 Waf1/Cip1 recombinant (12D1E1) Cell Signaling Cat# 2947; RRID: AB_823586 Anti-p21 Waf1/Cip1 Monoclonal (F-5) Santa Cruz Biotechnology Cat# sc-6246; RRID: AB_628073 anti-p16 INK4A Abcam Cat# ab189034; RRID: AB_2737282 anti-Ccng2 Invitrogen Cat# PA5-100707; RRID: AB_2850211 anti-GAPDH Abcam Cat# ab9485; RRID: AB_307275 anti-Alix Abcam Cat# ab275377; RRID: AB_3644262 anti-CD63 Abcam Cat# ab134045; RRID: AB_2800495 anti-TSG101 Abcam Cat# ab125011; RRID: AB_10974262 anti-Calnexin Abcam Cat# ab22595; RRID: AB_2069006 anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 anti-Ago2 Abcam Cat# ab186733; RRID: AB_2713978 anti-g-H2AX Cell Signaling Cat# 9718; RRID: AB_647828 anti-H3K9me3 Abcam Cat# ab8898; RRID: AB_306848 HRP anti-rabbit IgG Cell Signaling Cat# 7074; RRID: AB_2099233 HRP anti-mouse IgG Cell Signaling Cat# 7076; RRID: AB_330924 anti-Rabbit IgG H&L (Alexa Fluor 594) Abcam Cat# ab150080; RRID: AB_2650602 anti-Rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab15008; RRID: AB_301569 Chemicals, peptides, and recombinant proteins Doxorubicin Sigma Cat# 25316-40-9 Fetal bovine serum Gibco Cat# 10091-148 ncTarget Medium Nuwacell Biotechnologies Co., Ltd. Cat# RP01020 DAPI ThermoFisher Cat# D1306 EDTA ThermoFisher Cat# AM9261 DMEM ThermoFisher Cat# 11965092 Xho I New England Biolabs Cat# R0146S Not I New England Biolabs Cat# R0189S TRIzol Vazyme Cat# R401-01 FuGENE HD Promega Cat# E2311 Lipofectamine 2000 Life Technology Cat# 11668019 Protease inhibitor cocktail Cell Signaling Cat# 5871 Collagenase I Sigma Cat# SCR103 Collagenase IV Sigma Cat# C4-BIOC Dispase Sigma Cat# D4693 Red blood cell lysis buffer BD Biosciences Cat# 555899 Critical commercial assays Senescence b-Galactosidase Staining Kit Cell Signaling Cat# 9860 Quantibody Mouse TH17 Array 1 RayBiotech Cat# QAM-TH17-1 miRNeasy Mini Kit Qiagen Cat# 217004 Dual-Luciferase Reporter Assay Kit Promega Cat# E1910 Mouse IL-1b ELISA Kit R&D systems Cat# MLB00C Mouse IFN-g ELISA Kit R&D systems Cat# MIF00 Mouse IL-6 ELISA Kit R&D systems Cat# M6000B Mouse TNF-a ELISA Kit R&D systems Cat# MTA00B (Continued on next page) e1 Cell Metabolism 37, 1–15.e1–e6, February 4, 2025 .. REAGENT or RESOURCES SOURCE IDENTIFIER Deposited data scRNA-seq data This paper GEO: GSE247076 Public scRNA-seq data Almanzar et al.29 GEO: GSE132042 Bulk RNA-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Ago2 Clip-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Data S1.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Exosomal miR-302b rejuvenates aging mice by reversing the proliferative arrest of senescent cells.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-p21 Waf1/Cip1 recombinant (12D1E1) Cell Signaling Cat# 2947; RRID: AB_823586 Anti-p21 Waf1/Cip1 Monoclonal (F-5) Santa Cruz Biotechnology Cat# sc-6246; RRID: AB_628073 anti-p16 INK4A Abcam Cat# ab189034; RRID: AB_2737282 anti-Ccng2 Invitrogen Cat# PA5-100707; RRID: AB_2850211 anti-GAPDH Abcam Cat# ab9485; RRID: AB_307275 anti-Alix Abcam Cat# ab275377; RRID: AB_3644262 anti-CD63 Abcam Cat# ab134045; RRID: AB_2800495 anti-TSG101 Abcam Cat# ab125011; RRID: AB_10974262 anti-Calnexin Abcam Cat# ab22595; RRID: AB_2069006 anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 anti-Ago2 Abcam Cat# ab186733; RRID: AB_2713978 anti-g-H2AX Cell Signaling Cat# 9718; RRID: AB_647828 anti-H3K9me3 Abcam Cat# ab8898; RRID: AB_306848 HRP anti-rabbit IgG Cell Signaling Cat# 7074; RRID: AB_2099233 HRP anti-mouse IgG Cell Signaling Cat# 7076; RRID: AB_330924 anti-Rabbit IgG H&L (Alexa Fluor 594) Abcam Cat# ab150080; RRID: AB_2650602 anti-Rabbit IgG H&L (Alexa Fluor 488) Abcam Cat# ab15008; RRID: AB_301569 Chemicals, peptides, and recombinant proteins Doxorubicin Sigma Cat# 25316-40-9 Fetal bovine serum Gibco Cat# 10091-148 ncTarget Medium Nuwacell Biotechnologies Co., Ltd. Cat# RP01020 DAPI ThermoFisher Cat# D1306 EDTA ThermoFisher Cat# AM9261 DMEM ThermoFisher Cat# 11965092 Xho I New England Biolabs Cat# R0146S Not I New England Biolabs Cat# R0189S TRIzol Vazyme Cat# R401-01 FuGENE HD Promega Cat# E2311 Lipofectamine 2000 Life Technology Cat# 11668019 Protease inhibitor cocktail Cell Signaling Cat# 5871 Collagenase I Sigma Cat# SCR103 Collagenase IV Sigma Cat# C4-BIOC Dispase Sigma Cat# D4693 Red blood cell lysis buffer BD Biosciences Cat# 555899 Critical commercial assays Senescence b-Galactosidase Staining Kit Cell Signaling Cat# 9860 Quantibody Mouse TH17 Array 1 RayBiotech Cat# QAM-TH17-1 miRNeasy Mini Kit Qiagen Cat# 217004 Dual-Luciferase Reporter Assay Kit Promega Cat# E1910 Mouse IL-1b ELISA Kit R&D systems Cat# MLB00C Mouse IFN-g ELISA Kit R&D systems Cat# MIF00 Mouse IL-6 ELISA Kit R&D systems Cat# M6000B Mouse TNF-a ELISA Kit R&D systems Cat# MTA00B (Continued on next page) e1 Cell Metabolism 37, 1–15.e1–e6, February 4, 2025 .. REAGENT or RESOURCES SOURCE IDENTIFIER Deposited data scRNA-seq data This paper GEO: GSE247076 Public scRNA-seq data Almanzar et al.29 GEO: GSE132042 Bulk RNA-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Ago2 Clip-seq data Mendeley Data https://doi.org/10.17632/899tntr9x2.1 Data S1.



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